PARP-1 Knockout Immortalized Mouse Heart Endothelial (HYKO6) Cell Line | T3031
- 5 to 7 Days Shipment
abm | PARP-1 Knockout Immortalized Mouse Heart Endothelial (HYKO6) Cell Line | T3031
Poly(ADP-ribose) polymerase 1 (PARP-1) is an ADP-ribosylating enzyme that plays a critical role in DNA damage repair, in addition to functioning as a context- specific regulator of transcription factors. Various pathophysiological conditions, such as reperfusion injury, endotoxic shock, diabetes and aging are associated with endothelial cell dysfunction mediated by PARP-1.
9-10 weeks old 129/Sv x C57BL/6 mouse with PARP-1-/- genotype
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
CD105, CD31, ICAM-2, VCAM-1, vWF
For Research Use Only
The base medium for this cell line is Prigrow II medium available at abm
1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.
1) Flow cytometry was used to confirm the expression of endothelial cell markers such as CD105, CD31, ICAM-2, VCAM-1 and MHC Class I antigens; 2) Immunofluorescence staining was used to assess the vWF expression; 3) Western blot was used to evaluate the expression of SV40 T-antigen in the immortalized cell line.
10⁶ cells/1.0 ml