TLR3 Knockout Immortalized Mouse Dendritic Cell Line | T3034
- 5 to 7 Days Shipment
abm | TLR3 Knockout Immortalized Mouse Dendritic Cell Line | T3034
As messengers bridging the innate and adaptive immune system, the conventional tissue-resident DC (cDC) acts as sentinels in secondary lymphoid organs and other tissues for antigen capture and presentation. The MutuDCTlr3-/- cell line is an immortalized splenic CD8α+ subset (CD11chigh,B220-,DEC205+,CD24high,CD11b-) of conventional dendritic cells derived from the TLR3 knockout CD11c:SV40LgT transgenic mice .
CD11c:LgT-Tg x Tlr3(tm1.Flv) C57BL6 Mouse
20,000 – 60,000 cells/cm2.
Recommended split ratio: no greater than 1:3.
CD11c, CD24, MHC-II+, DEC205, Clec9A, B220, CD11b, IRF4, IRF8, CD4
For Research Use Only
The base medium for this cell line is IMDM 1x) + GlutamaxTM Gibco Ref: 31980-030). To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum
1. Freeze Medium: Complete growth medium with 50% decomplemented fetal calf serum (PAN biotech; P40-37500) and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.
1) Direct antigen presentation and cross-antigen presentation were evaluated by the MHC-I (SIINFEKL/OT-I ) and MHC-II (OVA323-339/OT-II) restricted systems; 3) proteome profile and surface markers assessed by RT-PCR; RT-PCR; 3) IL-12 cytokine secretion analyzed using ELISA ; 4) Response to PAMP stimulation evaluated by functional assays.
10⁶ cells/1.0 ml