Recombinant Human GRK1 (N-GST tag) | Z500154

abm | Recombinant Human GRK1 (N-GST tag) | Z500154

GRK1 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinases. GRK1 mediates rapid desensitization of rod photoreceptors to light by catalyzing phosphorylation of the visual pigment rhodopsin which leads to its deactivation . Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). GRK1 can be phosphorylated by PKA and this reduces the ability of GRK1 to phosphorylate rhodopsin in vitro . It is speculated that phosphorylation of GRK1 by PKA in vivo occurs in the dark when cAMP levels in photoreceptor cells are elevated.

Functions:

The specific activity of GRK1 was determined to be 4.3 nmol /min/mg as per activity assay protocol.

Organism:

Human

Source:

Insect cells

Alias:

GPRK1; RHOK; RK

Gene Symbol:

GRK1

Gene ID:

N/A

Accession:

NM_002929

Formulation:

50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Solubility:

N/A

Appearance:

Liquid

Molecular Weight (kDa):

95

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

70% - 90%

Concentration:

Shipping Condition :

Dry Ice

Storage Condition:

Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.