Recombinant Human MLK1 (N-GST tag) | Z500227

abm | Recombinant Human MLK1 (N-GST tag) | Z500227

MLK1 or Mixed-Lineage Kinase 1 is a mitogen-activated protein kinase kinase kinase capable of activating the c-Jun NH-terminal kinase (JNK) pathway. The catalytic domain of MLK1 has amino acid sequence similarity to both the tyr-specific and the ser/thr-specific kinase classes. In addition, MLK1 contain 2 leu/ile-zipper motifs and a basic sequence near its C-termini . MLK1 is threonine (and possibly serine) phosphorylated at multiple sites in the activation loop, with phosphorylation of Thr312 required for full activation .

Functions:

The specific activity of MLK1 was determined to be 116 nmol /min/mg as per activity assay protocol.

Organism:

Human

Source:

Insect cells

Alias:

MAP3K9, MEKK9; PRKE1

Gene Symbol:

MLK1

Gene ID:

N/A

Accession:

NM_033141

Formulation:

50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Solubility:

N/A

Appearance:

Liquid

Molecular Weight (kDa):

77

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

70% - 90%

Concentration:

Shipping Condition :

Dry Ice

Storage Condition:

Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.