Recombinant Human WNT1 | Z102125

abm | Recombinant Human WNT1 | Z102125

Wnt-1 is a secreted protein that signals through the frizzled family of seven transmembrane receptors. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the up-regulation of over fifty genes. Stabilization and resulting accumulation of the intracellular signaling protein β-catenin is an important aspect of Wnt-1 signaling. While known to play in role as a developmental protein, elevated levels of Wnt proteins have also been associated with tumorigenesis.

Functions:

Determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200ng/ml of human BMP-2.

Organism:

Human

Source:

E. coli

Alias:

Proto-oncogene Int-1 homolog, Wnt-1
Recombinant Human Proto-oncogene Wnt-1 (WNT1)

Gene Symbol:

WNT1

Gene ID:

7471

Accession:

P04628

Formulation:

Lyophilized from a 0.2 μm filtered solution in Tris and DTT

Solubility:

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1 - 1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (0.1% BSA) and store in working aliquots at -20 to -80⁰C

Appearance:

Lyophilized Powder

Molecular Weight (kDa):

38,5

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

>95% as determined by SDS-PAGE

Concentration:

<1.0 EU/μg of recombinant protein as determined by the LAL method

Shipping Condition :

Ambient Temperature

Storage Condition:

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.