Recombinant Rat IL6 | Z300089

abm | Recombinant Rat IL6 | Z300089

IL-6 is produced by many different cell types. The main sources in vivo are stimulated monocytes, fibroblasts, and endothelial cells. Macrophages, T cells and B lymphocytes, granulocytes, smooth muscle cells, eosinophils, chondrocytes, osteoblasts, mast cells, glial cells, and keratinocytes also produce IL-6 after stimulation. IL-6 is a member of a family of cytokines, which also includes LIF, CNTF, Oncostatin M, IL-11, and CT-1. All known members of the IL-6 cytokine family induce hepatic expression of acute phase proteins. The IL-6 receptor is expressed on T cells, mitogen-activated B cells, peripheral monocytes and some macrophage and B cell derived tumor cell types. It is not expressed in resting B cells but in resting T cells. The IL-6 receptor is a strongly glycosylated protein of 80 kDa and a length of 449 amino acids. It has been designated CD126.

Functions:

The ED(50) was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20-40 ng/mL.

Organism:

Rat

Source:

E. coli

Alias:

IL-6, IL6
Recombinant Rat Interleukin-6 (IL6)

Gene Symbol:

IL6

Gene ID:

24498

Accession:

P20607

Formulation:

Recombinant rat Interleukin-6 was lyophilized from a 0.2 μm filtered PBS solution.

Solubility:

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Appearance:

Lyophilized Powder

Molecular Weight (kDa):

22

Molecular Weight 2 (kDA):

N/A

Molecular Weight 3 (kDA):

N/A

Purity:

>95% as determined by SDS-PAGE

Concentration:

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Shipping Condition :

Ambient Temperature

Storage Condition:

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.