T4 DNA Polymerase | E005

abm | T4 DNA Polymerase | E005

T4 DNA Polymerase catalyzes the 5’→3’ synthesis of DNA from a single stranded, primed DNA template. This high fidelity enzyme also has potent 3’→5’ exonuclease activity but lacks 5’→3’ exonuclease function. T4 DNA Polymerase is the ideal choice for creating blunt ended DNA by removing 3’-overhangs or 5’-overhang filling, and is also useful for second strand DNA synthesis in site-specific mutagenesis.

Concentration:

5 U/μl

Form:

Enzyme supplied with 10X Reaction Buffer

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
This product is distributed for laboratory research only. Caution: Not for diagnostic use.

Application:

Restriction enzyme digestion of double-stranded DNA

Components:

NheI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)

Inactivation:

80°C for 20 min

Source:

An E. coli strain that carries the cloned and modified NheI gene from Neisseria mucosa heidelbergensis (ATCC 25999). The NheI enzyme recognizes and cuts the sequence G^CTAGC.

Shipping conditions:

Dry ice

Storage conditions:

Store all components at -20°C.

Storag Buffer:

100 mM KPO₄ (pH 6.5), 1 mM DTT, and 50% (v/v) Glycerol.