T4 DNA Polymerase | E005
- 5 to 7 Days Shipment
abm | T4 DNA Polymerase | E005
T4 DNA Polymerase catalyzes the 5’→3’ synthesis of DNA from a single stranded, primed DNA template. This high fidelity enzyme also has potent 3’→5’ exonuclease activity but lacks 5’→3’ exonuclease function. T4 DNA Polymerase is the ideal choice for creating blunt ended DNA by removing 3’-overhangs or 5’-overhang filling, and is also useful for second strand DNA synthesis in site-specific mutagenesis.
Enzyme supplied with 10X Reaction Buffer
One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Restriction enzyme digestion of double-stranded DNA
NheI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)
80°C for 20 min
An E. coli strain that carries the cloned and modified NheI gene from Neisseria mucosa heidelbergensis (ATCC 25999). The NheI enzyme recognizes and cuts the sequence G^CTAGC.
Store all components at -20°C.
100 mM KPO4 (pH 6.5), 1 mM DTT, and 50% (v/v) Glycerol.
500 U (100 μl)