E. coli DNA Polymerase I | G469

abm | E. coli DNA Polymerase I | G469

E.coli DNA Polymerase I is a DNA-dependent DNA polymerase with intrinsic 3’→5’ and 5’→3’ exonuclease activities. Nick translation is facilitated by this enzyme due to the removal of nucleotides ahead of the growing chain via 5´→3´ exonuclease activity.

Concentration:

5 U/μl

Form:

Enzyme supplied with 10X Reaction Buffer

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

Methylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked by Some Combinations of Overlapping

Application:

Reaction buffer for restriction enzyme digestion of double-stranded DNA

Components:

N/A

Inactivation:

N/A

Source:

N/A

Shipping conditions:

Dry ice

Storage conditions:

Store all components at -20°C.

Storag Buffer:

25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.