abm drug discovery
TLR4 & CD14 Stable Expressing CHO Cell Line (3E10 TLR4) | T6436
- SKU:
- T6436
- Availability:
- 5 to 7 Days Shipment
Description
abm | TLR4 & CD14 Stable Expressing CHO Cell Line (3E10 TLR4) | T6436
Toll-like receptor 4 is a signal transducer that is known to implicate cellular responses to lipopolysaccharides. To generate the TLR4 & CD14 Stable Expressing CHO Cell Line (3E10 TLR4) CHO/CD14 reporter cells were stably transfected with the human cDNA for TLR4 in the pFLAG-CMV-1 vector.
Biosafety:
II
Organism:
Chinese Hamster (C. griseus)
Source Organ:
Ovary
Growth Properties:
Adherent
Morphology:
Epithelial-Like
Clones:
N/A
Passage Number:
N/A
Population Doner:
20 - 30 hours
Seeding Density:
20,000 - 40,000 cells/cm2
Markers:
Inducible membrane CD25 under the control of nuclear factor-κB (NF-κB) and Toll-like Receptor 4 Hygromycin B, Ciprofloxacin, and Puromycin Resistant
Applications:
For Research Use Only
Doner Gender:
N/A
Donor Ethnicity:
N/A
Knockdown Method:
N/A
Induction:
N/A
Overexpression:
N/A
Freeze Thaw:
N/A
Propagation:
Preservation:
1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control:
Flow cytometry using a FACScan microfluorometer & Western Blot
Tumorgenicn:
N/A
Shipping Conditions:
Dry Ice
Storage Contidions:
-180°C