CAP1 Stable Knockdown AsPC-1 Cell Line | T6403

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SKU:
T6403
Availability:
5 to 7 Days Shipment
€4,173.00
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Description

abm | CAP1 Stable Knockdown AsPC-1 Cell Line | T6403

CAP1 mediate extracellular signals to control cancer cell invasiveness. Using two shRNA constructs S2 and S3 that target independent nucleotide sequences and effectively silenced CAP1 in HeLa and breast cancer cells, we were able to generate stable clones with efficient CAP1 knockdown in the PANC-1 and AsPC-1 cells, as confirmed in Western blotting. Knockdown of CAP1 in cancer cells led to enhanced stress fibers, reduced cell motility and invasion into Matrigel. These cells can be used to investigated roles for CAP1 and its phosphor-regulation in pancreatic cancer cells.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Pancreas

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

50,000 cells/cm2

Markers:

AsPC-1 (clone S2-3) Drug resistance: 250 ug/ml neomycin Vector information: pRNA-U6.1/Neo vector was used S2 targets nucleotide 519-537 of human CAP1 gene; S3 targets nucleotides 1074-1092 of human CAP1 gene.

Applications:

For Research Use Only

Doner Gender:

Female

Donor Ethnicity:

Caucasian

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

Use of PriCoatTM T25 Flasks

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Western blot

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C

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Additional Information

Size:
10⁶ cells/1.0 ml
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