CAP1 Stable Knockdown PANC-1 Cell Line | T6404

abm | CAP1 Stable Knockdown PANC-1 Cell Line | T6404

CAP1 mediate extracellular signals to control cancer cell invasiveness. Using two shRNA constructs S2 and S3 that target independent nucleotide sequences and effectively silenced CAP1 in HeLa and breast cancer cells, we were able to generate stable clones with efficient CAP1 knockdown in the PANC-1 and AsPC-1 cells, as confirmed in Western blotting. Knockdown of CAP1 in cancer cells led to enhanced stress fibers, reduced cell motility and invasion into Matrigel. These cells can be used to investigated roles for CAP1 and its phosphor-regulation in pancreatic cancer cells.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Pancreas

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

31 - 41 hours

Seeding Density:

20,000 - 30,000 cells/cm²

Markers:

PANC-1 (clone S3-3) Drug resistance: 250 ug/ml neomycin Vector information: pRNA-U6.1/Neo vector was used S2 targets nucleotide 519-537 of human CAP1 gene; S3 targets nucleotides 1074-1092 of human CAP1 gene.

Applications:

For Research Use Only

Doner Gender:

Male

Donor Ethnicity:

Caucasian

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation: