DNA dA-Tailing Kit | E009

abm | DNA dA-Tailing Kit | E009

The DNA dA-Tailing Kit efficiently adds a non-template dAMP (dA) to the 3’ end of a blunt-ended DNA fragment. This incorporated 3’-dA prevents concatemer formation and prepares the DNA fragment for subsequent ligation of adaptors or cloning vectors that have complementary 3’-dT overhangs. The Klenow Fragment (3´→ 5´ Exo-) in this kit adds the 3’-dA via its DNA polymerase activity. This enzyme lacks 5’→3’ exonuclease activity and has mutations that effectively abolish the inherent 3’→5’ exonuclease activity, thus preventing degradation of this 3’-dA. This kit has been optimized to prepare compatible overhangs for the next step of DNA sample preparation for next generation sequencing. The Klenow Fragment (3´→ 5´ Exo-) enzyme is also available as a stand-alone enzyme (Cat. No. E038).

Concentration:

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Form:

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Alternate Name:

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Reaction Buffer:

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Reaction:

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Notes:

One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments.

This product does not contain any RNase inhibitors, so it is recommended to be used in combination with RNaseOFF Ribonuclease Inhibitor (Cat. G138). This product should not be used in digestions longer than 15 minutes or at temperatures higher than 37°C, or the residual RNase activity will begin to degrade the RNA.

This product is distributed for laboratory research only
Caution: Not for diagnostic use.

Application:

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Components:

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Inactivation:

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Source:

E. coli

Shipping conditions:

Dry ice

Storage conditions:

Store all components at -20°C in a non-frost-free freezer. All components are stable for 1 year from the date of shipping when stored and handled properly.

Storag Buffer:

25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.