Immortalized Mouse Cardiomyogenic Progenitor Cells (iCP) | T0449

abm | Immortalized Mouse Cardiomyogenic Progenitor Cells (iCP) | T0449

abm Cat. No. 000018A) to revert immortal cells back into primary cells resulting in increasing differentiation potential. In the presence of retinoic acid, the cells undergo cardiomyogenesis where late cardiomyogenic markers (Actc1 and ANF) will be present. Dexamethasone may be used as a differentiation reagent, to express late markers ANF, cTnT, and α-MyHC. These cells are recommended for studies relating to cardiomyogenic progenitors in basic and/or translational research.

Immortalization Method:

Serial passaging and transduction with retrovirus SSR#69 expressing SV40 large T antigen flanked with Cre/loxP sites

BioSafety Level:

II

Organism:

CD1 Mouse Embryo

Species:

Mouse

Source Organ:

Heart

Organ Type:

Heart

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) RT-PCR, qPCR, and sqPCR to detect cell markers; 2) Western blot analysis for SV40 large T antigen presence.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C