Immortalized Mouse Dendritic Cells (MutuDC1940) | T0528
- 5 to 7 Days Shipment
abm | Immortalized Mouse Dendritic Cells (MutuDC1940) | T0528
The MutuDC1940 cell line retains response to TLR ligands such as CpG (TLR9-L) and PolyIC (TLR3-L) and to a lesser extent LPS (TLR4-L) stimulation by up-regulation of co-stimulatory molecules CD40, CD80 and CD86. In addition to responding to PAMP stimulation and producing Th1 cytokines such as IL-12, these cells are also capable of presenting antigen in the context of both MHC-I and MHC-II, including direct antigen presentation and cross-presentation of cell-associated antigens. Together with TLR3 knockout (Cat. No. T3034), TLR9 knockout (Cat. No. T3035) and Ifnar1 knockout (Cat. No. T3036) MutuDC, these dendritic cell lines provide a powerful tool in vaccine science and immunotherapy, particularly in strategizing target antigens to CD8α+ subset.
Isolated from C57BL/6 transgenic mice carrying SV40 Large T oncogene
CD11c:SV40LgT-transgenic C57BL/6 mice
45 - 55 hours
20,000 – 60,000 cells/cm10².
Recommended split ratio: no greater than 1:3.
CD11c, CD24, MHC-II+, DEC205, Clec9A, B220, CD11b, IRF4, IRF8, CD4
The base medium for this cell line is IMDM (1x) + GlutamaxTM (Gibco Ref: 31980-030). To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%, 1% of 7.5% Sodium Bicarbonate Solution, 50 µM β-mercaptoethanol, HEPES to a final concentration of 10 mM, and Penicillin/Streptomycin Solution to a final concentration of 1%. Filter the complete media at 0.22µm before use.Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
1) Direct antigen presentation and cross-antigen presentation were evaluated by the MHC-I (SIINFEKL/OT-I ) and MHC-II (OVA323-339/OT-II) restricted systems; 3) proteome profile and surface markers assessed by RT-PCR; RT-PCR; 3) IL-12 cytokine secretion analyzed using ELISA ; 4) Response to PAMP stimulation evaluated by functional assays.
liquid nitrogen or -180C
1x10⁶ cells / 1.0 ml