Immortalized Mouse Tumour Dendritic Cells (CD4⁻ MutuDC2) | T0790

abm | Immortalized Mouse Tumour Dendritic Cells (CD4⁻ MutuDC2) | T0790

hiand CD86^hi. These observations demonstrate that the CD4⁻ MutuDC2s have a relatively mature splenic resident conventional DC1 phenotype. They can be easily manipulated with standard experimental techniques and display great stability of their phenotypic and functional properties in long term culture as long as their passage number is maintained below 40-50. For these reasons the CD4⁻ MutuDC2s represent a new valuable tool for the study of dendritic cell biology.

Immortalization Method:

Spontaneous SV40LgT-mediated tumorigenic transformation of splenic conventional Dendritic Cells in CD11c-SV40LgT-transgenic mice.

BioSafety Level:

II

Organism:

Mouse

Species:

Mouse

Source Organ:

Spleen

Organ Type:

Spleen

Growth Properties:

Adherent

Morphology:

Round

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

72-144 hours

Markers:

CD11c+, MHC-II+, F4/80lo, B220-, GR-1-, PDCA-1-, CD80+, CD86+, CD11b+, CD172a+, CD4-, CD8a-, CLEC9A-, FLT3+

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is IMDM (1x) + Glutamax^TM (Gibco Ref: 31980-030). To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%, 1% of 7.5% Sodium Bicarbonate Solution, 50 µM β-mercaptoethanol, HEPES to a final concentration of 10 mM, and Penicillin/Streptomycin Solution to a final concentration of 1%. Filter the complete media at 0.22µm before use.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

To decomplement FBS:
1. Let the FBS bottle completely thaw overnight at 4°C.
2. Leave the FBS bottle in water bath for 30 minutes at 56°C.
3. Decomplemented FBS can be stored at -20°C for long term. Avoid frequent freeze-thaw cycling.

Quality Control:

1) Flow cytometry; 2) ELISA; 3) qPCR

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C