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Immortalized Mouse Olfactory Placode Cells (OP6) | T0786

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SKU:
T0786
Availability:
5 to 7 Days Shipment
€4,500.00
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Description

abm | Immortalized Mouse Olfactory Placode Cells (OP6) | T0786

Immortalization Method:

Immortalized via retroviral transduction of the U19-tsA58 TAg simian virus 40 large tumor antigen

BioSafety Level:

II

Organism:

Mouse (M. musculus)

Species:

Mouse

Source Organ:

Embryo

Organ Type:

Embryo

Growth Properties:

Adherent

Morphology:

Tear-shaped

Passage Number:

N/A

Population Doubling:

Approximately 99 hours

Seeding Density:

N/A

Markers:

Neomycin resistance

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

Use of PriCoatTM T25 Flasks or Applied Cell Extracellular Matrix is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is Prigrow III medium available at abm. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 33.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Southern blot analysis; 2) RT-PCR

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C

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Additional Information

Size:
1x10⁶ cells / 1.0 ml
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