Ovine Brown Adipocyte Precursor Cell Line | T6311

abm | Ovine Brown Adipocyte Precursor Cell Line | T6311

Brown adipose tissue (BAT) plays a critical role in regulating body temperature. Ovine brown adipocyte precursor cells (BAPC) were isolated from fetal labs at (d 90) of gestation and established into a stable cell line. The cells are characterized by adipogenic differentiation and expression of hallmark genes, uncoupling protein 1 (UCP1). These cells present us with an opportunity to study the molecular mechanism behind the nutritional regulation of BAT growth and development in fetus.

Biosafety:

II

Organism:

Sheep (Ovis)

Source Organ:

Adipose

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 hours

Seeding Density:

N/A

Markers:

Uncoupling protein 1 (UCP1)

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm

Preservation:

1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Oil Red-O staining , 2) Reverse-transcription PCR (RT-PCR), 3) Western blot

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C