Description
abm | Poly(A) Polymerase, Yeast | E017
Poly(A) Polymerase, Yeast catalyses the template independent addition of adenosine residues onto the 3' ends of polyribonucleotides. The use of ATP as a substrate leads to poly(A) tailing whereas substitution of cordycepin-5'-triphosphate (3'-dATP) for ATP results in addition of a single dA residue to the 3’-termini of the RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast has been shown to be more effective at oligonucleotide-labeling and poly(A) tailing of long RNA templates than Poly(A) Polymerase from E. coli.
Concentration:
1 U/μl
Form:
N/A
Alternate Name:
N/A
Reaction Buffer:
N/A
Reaction:
N/A
Notes:
N/A
Application:
Restriction enzyme digestion of double-stranded DNA
Components:
EcoRI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Inactivation:
65°C for 20 min
Source:
An E. coli strain that carries the modified EcoRI gene from E. coli RY13. The EcoRI enzyme recognizes and cuts the sequence G^AATTC.
Shipping conditions:
Dry ice
Storage conditions:
Store all components at -20°C.
Storag Buffer:
20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton® X-100 and 50% (v/v) Glycerol.