T7 RNA Polymerase | E041

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SKU:
E041
Availability:
5 to 7 Days Shipment
€132.00
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Description

abm | T7 RNA Polymerase | E041

T7 RNA Polymerase is a DNA dependent RNA polymerase that catalyzes the synthesis of RNA in the 5´→ 3´ direction only in the presence of its cognate T7 phage promoter sequence. T7 RNA Polymerase has high specificity for the T7 phage promoter and will not recognize SP6 or T3 RNA Polymerase promoter sequences.

Concentration:

50 U/ul

Form:

N/A

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

One unit is defined as the amount of T7 RNA Polymerase that is required to incorporate 1 nmol ATP into acid-insoluble material in a 50 μl reaction volume in 1 hour at 37°C in 1X T7 RNA Polymerase Reaction Buffer.

Application:

  • transformation efficiency: >1x10^6 cfu/µg with abm’s expression vectors (>10kb in size).
  • ideal for routine plasmid amplification.
  • reduces recombination and improves yield and quality of plasmid DNA prepared from minipreps; can be sequenced directly.
  • not Suitable for preparing unmethylated DNA.

    Components:

    N/A

    Inactivation:

    N/A

    Source:

    E. coli

    Shipping conditions:

    Dry ice

    Storage conditions:

    Store all components at -20°C.

    Storag Buffer:

    50 mM Tris-HCl (pH 8), 1 mM EDTA, 20 mM β-ME, 100 mM NaCl, 0.1% Triton®X-100 and 50% (v/v) Glycerol.

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    Additional Information

    Size:
    100 μl (5000 U)
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