RNase H, E. coli | E018

abm | RNase H, E. coli | E018

RNase H (Ribonuclease H), E. coli is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA strands in RNA-DNA hybrids. This enzyme cleaves the 3'-O-P-bond of RNA and generates 3' hydroxyl and 5' phosphate products. RNase H does not digest single-stranded or double-stranded DNA and RNA.

Concentration:

5 U/μl

Form:

Enzyme supplied with 10X Reaction Buffer

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

One unit is defined as the amount of RNase H, E. coli that is required to hydrolyze 1 nmol of the RNA in radiolabeled poly(rA):poly(dT), to acid-soluble ribonucleotides in a total reaction volume of 50 μl in 20 minutes at 37°C in 1X RNase H, E. coli Reaction Buffer with 10 nmol radiolabeled poly(rA) and 12.5 μg poly(dT).
This product is distributed for laboratory research only. Caution: Not for diagnostic use.

Application:

Restriction enzyme digestion of double-stranded DNA

Components:

HaeIII enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204).

Inactivation:

80°C for 20 min

Source:

An E. coli strain that carries the cloned and modified HaeIII gene from Haemophilus aegypticus (ATCC 11116). The HaeIII enzyme recognizes and cuts the sequence GG^CC.

Shipping conditions:

Dry ice

Storage conditions:

Store all components at -20°C.

Storag Buffer:

20 mM Tris-HCl (pH 7.5), 100 mM KCl, 10 mM MgCl₂, 0.1 mM DTT, 0.1 mM EDTA, and 50% (v/v) Glycerol.