E. coli DNA Ligase | E007
- 5 to 7 Days Shipment
abm | E. coli DNA Ligase | E007
E.coli DNA Ligase is an NAD+-dependent enzyme that catalyzes the formation of a phosphodiester bond between cohesive 3′-hydroxyl and 5′-phosphoryl termini of double-stranded DNA (dsDNA). This enzyme is also active on nicked DNA but is not effective for the formation of DNA-RNA or RNA-RNA hybrids.
Enzyme supplied with 10X Reaction Buffer
Reaction buffer for restriction enzyme digestion of double-stranded DNA
Store all components at -20°C.
50 mM Tris-HCl (pH 7.5), 1 mM EDTA, 1 mM DTT, 200 mM NaCl, and 50% (v/v) Glycerol.
2000 U (100 μl)