SIRT7 Stable Knockdown H1299 Cell Line | T6165
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abm | SIRT7 Stable Knockdown H1299 Cell Line | T6165
The sirtuin family of proteins have a diverse range of biological functions, accomplished by the deacetylation of their targets. Derived from H1299 cells, this Sirt7 knockdown cell line was displays lentiviral-driven stable expression shRNA scramble sequences and a Sirt7 specific shRNA. These cells would be suitable for use in the study of the functions of Sirt7 and the sirtuin family of proteins
Human (H. sapiens)
43 - 53 hours
10,000 - 20,000 cells/cm2
Puromycin resistance at the concentration of 5 µg/mL. The cells stably express the short hairpin RNA (shRNA) scramble sequence: 5′-CCTAAGGTTAAGTCGCCCTCGCTCGAGCGAGGGCGACTTAACCTT AGG-3′ and the Sirt7 specific shRNA 5′- CCGGGTCCAGCCTGAAGGTTCTAAACTCGAGTTTAGAACCTTCAGGCTGGACTTTTTG-3′.
For Research Use Only
The base medium for this cell line is Prigrow III medium available at abm
1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
1) Immunofluorescence; 2) DNA analysis and qPCR; 3) AgNOR staining
10⁶ cells/1.0 ml