T7 Endonuclease I | E028

abm | T7 Endonuclease I | E028

T7 Endonuclease I recognizes and cleaves mismatched DNA, heteroduplex DNA, cruciform DNA, Holliday structures and/or junctions, as well as nicked double-stranded DNA. This enzyme has a preference for single stranded over double stranded DNA with cleavage occurring at the first, second or third phosphodiester bond that is 5´ to the mismatch.

Concentration:

10 U/μl

Form:

Enzyme supplied with 10X Reaction Buffer

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

One unit is defined as the amount of T7 Endonuclease I that linearizes over 90% of 1 µg of supercoiled cruciform DNA in a 50 µl volume in 1 hour at 37°C.

Application:

1. Rapid multi-fragment cloning and assembly of whole vector constructs
2. Clone in a promoter, gene, and tag, all in one step
3. Rapid assembly of CRISPR multiplex gRNA vectors in one step

Components:

N/A

Inactivation:

N/A

Source:

N/A

Shipping conditions:

Dry ice

Storage conditions:

Store all components at -20°C. Avoid repeated freeze-thaw cycles of all components to retain maximum performance. All components are stable for 1 year from the date of shipping when stored and handled properly.

Storag Buffer:

25 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.15% Triton® X-100 and 50% (v/v) Glycerol.