T7 Endonuclease I | E028
- 5 to 7 Days Shipment
abm | T7 Endonuclease I | E028
T7 Endonuclease I recognizes and cleaves mismatched DNA, heteroduplex DNA, cruciform DNA, Holliday structures and/or junctions, as well as nicked double-stranded DNA. This enzyme has a preference for single stranded over double stranded DNA with cleavage occurring at the first, second or third phosphodiester bond that is 5´ to the mismatch.
Enzyme supplied with 10X Reaction Buffer
One unit is defined as the amount of T7 Endonuclease I that linearizes over 90% of 1 µg of supercoiled cruciform DNA in a 50 µl volume in 1 hour at 37°C.
- Rapid multi-fragment cloning and assembly of whole vector constructs
- Clone in a promoter, gene, and tag, all in one step
- Rapid assembly of CRISPR multiplex gRNA vectors in one step
Store all components at -20°C. Avoid repeated freeze-thaw cycles of all components to retain maximum performance. All components are stable for 1 year from the date of shipping when stored and handled properly.
25 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.15% Triton® X-100 and 50% (v/v) Glycerol.
1000 U (100 μl)