null

Uracil DNA Glycosylase | E080

(No reviews yet) Write a Review
SKU:
E080
Availability:
5 to 7 Days Shipment
€81.25
Frequently bought together:

Description

abm | Uracil DNA Glycosylase | E080

E. coli Uracil DNA Glycosylase (UDG) catalyses the release of free uracil via hydrolysis of the N-glycosylic bond between the uracil and sugar from uracil containing single- or double- stranded DNA. This enzyme does not show activity on oligomers of 6 or less bases or on RNA.

Concentration:

5 U/μl

Form:

Enzyme supplied with 10X Reaction Buffer.

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

One unit is defined as the amount of Uracil DNA Glycosylase that catalyzes the release of 60 pmol of uracil per one minute from uracil-containing, double-stranded DNA at 37°C.
This product is distributed for laboratory research only.
Caution: Not for diagnostic use.

Application:

  • displacement loop mutagenesis
  • targeted DNA cleavage
  • visualization of DNA with electron microscopy
  • library screening with RecA coated probes

    Components:

    N/A

    Inactivation:

    N/A

    Source:

    N/A

    Shipping conditions:

    Ice Packs

    Storage conditions:

    Store all components at -20 °C.

    Storag Buffer:

    30 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.15% Tween-20 and 50% (v/v) Glycerol.

  • View AllClose

    Additional Information

    Size:
    1000 U (200 μl)
    View AllClose