Taq DNA Ligase | E090
- 5 to 7 Days Shipment
abm | Taq DNA Ligase | E090
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between adjacent 5’-phosphate and 3’-hydroxyl groups of duplex DNA. This enzyme is a NAD+ dependent DNA ligase.
Enzyme supplied with a 10X Reaction Buffer.
Incubate at 45°C.
One unit is defined as the amount of enzyme required to give 50% ligation of the 12 bp cohesive ends of 1 µg of BstEII-digested λ DNA in a total reaction volume of 50 µl in 15 min at 45°C.
Taq DNA Ligase cannot be used as a substitute for T4 DNA Ligase.
This product is distributed for laboratory research only.
Caution: Not for diagnostic use.
Restriction enzyme digestion of double-stranded DNA
PstI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)
An E. coli strain that carries the cloned and modified PstI gene from Providencia stuartii164 (ATCC 49762). The PstI enzyme recognizes and cuts the sequence CTGCA^G.
Store all components at -20°C upon arrival. Avoid repeated freeze-thaw cycles of all components to retain maximum performance. For long term storage, store the enzyme at -20°C, and the reaction buffer at -80°C to extend the half life of NAD+.
10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol.
10000 U (250 µl)