T4 DNA Ligase | G467

abm | T4 DNA Ligase | G467

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3’- hydroxyl and 5’- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.

Concentration:

1 U/μl

Form:

Enzyme supplied with 5X Reaction Buffer

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

This product is distributed for laboratory research only. Caution: Not for diagnostic use.

Application:

Restriction enzyme digestion of double-stranded DNA

Components:

NdeI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)

Inactivation:

No

Source:

An E. coli strain that carries the NdeI gene from Neisseria denitrificans (NRCC 31009). The NdeI enzyme recognizes and cuts the sequence CA^TATG.

Shipping conditions:

Dry ice

Storage conditions:

Store all components at -20°C.

Storag Buffer:

N/A