T4 DNA Ligase | G467
- 5 to 7 Days Shipment
abm | T4 DNA Ligase | G467
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3’- hydroxyl and 5’- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.
Enzyme supplied with 5X Reaction Buffer
This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Restriction enzyme digestion of double-stranded DNA
NdeI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)
An E. coli strain that carries the NdeI gene from Neisseria denitrificans (NRCC 31009). The NdeI enzyme recognizes and cuts the sequence CA^TATG.
Store all components at -20°C.
200 U (200 μl)