T4 RNA Ligase 1 (ssRNA Ligase) | E010

abm | T4 RNA Ligase 1 (ssRNA Ligase) | E010

T4 RNA Ligase 1 catalyzes the ATP-dependent formation of a 3'→5' phosphodiester bond on a broad range of substrates including single-stranded RNA and DNA, oligoribonucleotides, oligodeoxynucleotides, as well as numerous nucleotide derivatives. The phosphodiester bond is created between a 5′-phosphoryl-terminated nucleic acid donor and a 3′-hydroxyl-terminated nucleic acid acceptor in a template-independent manner.

Concentration:

10 U/μl

Form:

Enzyme supplied with 10X Reaction Buffer

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

One unit is defined as the amount of T4 RNA Ligase 1 that converts 1 nmole of 5´-[³²P]rA₁₆ into a phosphatase-resistant form in 30 minutes at 37°C.
This product is distributed for laboratory research only.
Caution: Not for diagnostic use.

Application:

• Synthesis of RNA transcripts for hybridization probes
• Synthesis of RNA for in vitro translation
• Synthesis of biologically active RNA like mRNA or lncRNA
• Generate large amounts of labelled or non-labelled RNA

Components:

N/A

Inactivation:

N/A

Source:

N/A

Shipping conditions:

Dry ice

Storage conditions:

Store all components at -20°C.

Storag Buffer:

10 mM Tris-HCl (pH 7.5), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT and 50% (v/v) Glycerol.