T4 RNA Ligase 2 (Truncated) | E012

abm | T4 RNA Ligase 2 (Truncated) | E012

T4 RNA Ligase 2 (Truncated) catalyzes the formation of a phosphodiester bond between a pre-adenylated 5’ phosphate (DNA or RNA) and the 3’ hydroxyl of RNA. This truncated enzyme contains the first 249 amino acids and does not require ATP for ligation but does require a pre-adenylated 5’ terminal donor. In cases where the substrate is pre-adenylated on the 5’ end, T4 RNA ligase 2 (Truncated) is a better choice compared to the full-length enzyme because it generates less side-reaction ligation products as it cannot ligate the phosphorylated 5´ end of RNA or DNA to the 3´ end of RNA.

Concentration:

200 U/μl

Form:

Enzyme supplied with 10X Reaction Buffer

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

One unit is defined as the amount of T4 RNA Ligase 2 (Truncated) required to ligate 50% of 0.4 μg of an equimolar mix of a single-stranded 5’ FAM-labelled 17-mer RNA to the 5’ pre-adenylated end of a 18-mer DNA when both 17-mers are annealed to a complementary 35-mer DNA strand in a total reaction volume of 20 µl in 30 minutes at 37°C.
This product is distributed for laboratory research only. Caution: Not for diagnostic use.

Application:

• Labelling of RNA with ATP or cordycepin • Poly(A) tailing of RNA for cloning or affinity purification • Increasing translation of RNA transferred into eukaryotic cells

Components:

Enzyme supplied with 5X Reaction Buffer.

Inactivation:

N/A

Source:

N/A

Shipping conditions:

Dry ice

Storage conditions:

Store all components at -20°C.

Storag Buffer:

10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT and 50% (v/v) Glycerol.