Taq DNA Ligase | E090

abm | Taq DNA Ligase | E090

Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between adjacent 5’-phosphate and 3’-hydroxyl groups of duplex DNA. This enzyme is a NAD+ dependent DNA ligase.

Concentration:

40000 U/ml

Form:

Enzyme supplied with a 10X Reaction Buffer.
Incubate at 45°C.

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

One unit is defined as the amount of enzyme required to give 50% ligation of the 12 bp cohesive ends of 1 µg of BstEII-digested λ DNA in a total reaction volume of 50 µl in 15 min at 45°C.

Taq DNA Ligase cannot be used as a substitute for T4 DNA Ligase.

This product is distributed for laboratory research only.
Caution: Not for diagnostic use.

Application:

Restriction enzyme digestion of double-stranded DNA

Components:

PstI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204)

Inactivation:

No

Source:

An E. coli strain that carries the cloned and modified PstI gene from Providencia stuartii164 (ATCC 49762). The PstI enzyme recognizes and cuts the sequence CTGCA^G.

Shipping conditions:

Dry ice

Storage conditions:

Store all components at -20°C upon arrival. Avoid repeated freeze-thaw cycles of all components to retain maximum performance. For long term storage, store the enzyme at -20°C, and the reaction buffer at -80°C to extend the half life of NAD+.

Storag Buffer:

10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol.