E. coli DNA Ligase | E007

abm | E. coli DNA Ligase | E007

E.coli DNA Ligase is an NAD+-dependent enzyme that catalyzes the formation of a phosphodiester bond between cohesive 3′-hydroxyl and 5′-phosphoryl termini of double-stranded DNA (dsDNA). This enzyme is also active on nicked DNA but is not effective for the formation of DNA-RNA or RNA-RNA hybrids.

Concentration:

10 U/μl

Form:

Enzyme supplied with 10X Reaction Buffer

Alternate Name:

N/A

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

N/A

Application:

Reaction buffer for restriction enzyme digestion of double-stranded DNA

Components:

N/A

Inactivation:

N/A

Source:

N/A

Shipping conditions:

Dry ice

Storage conditions:

Store all components at -20°C.

Storag Buffer:

50 mM Tris-HCl (pH 7.5), 1 mM EDTA, 1 mM DTT, 200 mM NaCl, and 50% (v/v) Glycerol.