Molecular Biology and PCR

abm | HindIII | E071 N/AConcentration: 20000 U/mlForm: N/AAlternate Name: N/AReaction Buffer: Buffer 1: 10%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100%View the buffer compatibility chart for restriction enzymes.Reaction: 1X Universal...

abm | HaeIII | E070 N/AConcentration: 10000 U/mlForm: N/AAlternate Name: N/AReaction Buffer: Buffer 1: 50%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100%View the buffer compatibility chart for restriction enzymes.Reaction: 1X Universal...

abm | HaeII | E060 N/AConcentration: 20000 U/mlForm: N/AAlternate Name: N/AReaction Buffer: Buffer 1: 25%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100% Click here to view the buffer compatibility chart for restriction enzymes.Reaction: 1X...

abm | Exonuclease III, E. coli | E016 Exonuclease III, E.coli digests duplex DNA in the 3´→5´ direction from nicked DNA, blunt end DNA, 3´-recessed ends, or 3´-overhangs of less than four bases, and yields nucleoside 5´-phosphates. The DNA degradation...

abm | Exonuclease I, E. coli | E015 Exonuclease I, E.coli catalyzes the removal of nucleotides from single-stranded DNA in the 3’→5’ direction, releasing deoxyribonucleoside 5'-monophosphates and leaving 5'-terminal dinucleotides intact. Hydrolysis...

abm | EcoRV | E059 N/AConcentration: 20000 U/mlForm: N/AAlternate Name: N/AReaction Buffer: Buffer 1: 25%, Buffer 2: 100%, Buffer 3: 100%, Universal Buffer: 100%Click here to view the buffer compatibility chart for restriction enzymes.Reaction: 1X...

abm | EcoRI | E048 N/AConcentration: 20000 U/mlForm: N/AAlternate Name: N/AReaction Buffer: Buffer 1: 10%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100%Click here to view the buffer compatibility chart for restriction enzymes.Reaction: 1X...

abm | E. coli Single-Stranded Binding Protein | E022 N/AConcentration: 1mg/mlForm: N/AAlternate Name: N/AReaction Buffer: N/AReaction: N/ANotes: Methylation Sensitivity dam methylation: Not Sensitivedcm methylation: Not SensitiveCpG Methylation: Blocked...

abm | E. coli DNA Polymerase I | G469 E.coli DNA Polymerase I is a DNA-dependent DNA polymerase with intrinsic 3’→5’ and 5’→3’ exonuclease activities. Nick translation is facilitated by this enzyme due to the removal of nucleotides ahead of the growing...

abm | E. coli DNA Ligase | E007 E.coli DNA Ligase is an NAD+-dependent enzyme that catalyzes the formation of a phosphodiester bond between cohesive 3′-hydroxyl and 5′-phosphoryl termini of double-stranded DNA (dsDNA). This enzyme is also active on...

abm | DpnI | E026 The DpnI restriction enzyme digests DNA at GmeA↓TC sites, requiring N6-methylation of the adenine residue for activity. DNA purified from a dam+ strain will be a substrate for DpnI due to the adenine methylation. DpnI cleaves...

abm | DNase I (RNAse-Free) | E091 Deoxyribonuclease I (DNase I; EC 3.1.21.1) is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA-DNA hybrids. DNase I cleaves DNA to release...