Molecular Biology and PCR

abm | Glycogen | G024 An analogue of starch, glycogen is used in many areas of research in order to recover nucleic acids. It is possible to obtain nanogram or picogram quantities of DNA and RNA by using glycogen.Concentration: N/AGrade: N/AComposition:...

abm | XbaI | E052 N/AConcentration: 20000 U/mlForm: N/AAlternate Name: N/AReaction Buffer: Buffer 1: 10%, Buffer 2: 100%, Buffer 3: 75%, Universal Buffer: 100%Click here to view the buffer compatibility chart for restriction enzymes.Reaction: 1X...

abm | Uracil DNA Glycosylase | E080 E. coli Uracil DNA Glycosylase (UDG) catalyses the release of free uracil via hydrolysis of the N-glycosylic bond between the uracil and sugar from uracil containing single- or double- stranded DNA. This enzyme does...

abm | Taq DNA Ligase | E090 Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between adjacent 5’-phosphate and 3’-hydroxyl groups of duplex DNA. This enzyme is a NAD+ dependent DNA ligase.Concentration: 40000...

abm | T7 RNA Polymerase | E041 T7 RNA Polymerase is a DNA dependent RNA polymerase that catalyzes the synthesis of RNA in the 5´→ 3´ direction only in the presence of its cognate T7 phage promoter sequence. T7 RNA Polymerase has high specificity for the...

abm | T7 Endonuclease I | E028 T7 Endonuclease I recognizes and cleaves mismatched DNA, heteroduplex DNA, cruciform DNA, Holliday structures and/or junctions, as well as nicked double-stranded DNA. This enzyme has a preference for single stranded over...

abm | T5 Exonuclease | E089 T5 exonuclease catalyzes the degradation of nucleotides either from the 5’ termini or at nicks of linear or circular dsDNA in a 5’ to 3’ direction. This exonuclease also exhibits ssDNA endonuclease activity in the presence of...

abm | T4 RNA Ligase 2 (Truncated) | E012 T4 RNA Ligase 2 (Truncated) catalyzes the formation of a phosphodiester bond between a pre-adenylated 5’ phosphate (DNA or RNA) and the 3’ hydroxyl of RNA. This truncated enzyme contains the first 249 amino acids...

abm | T4 RNA Ligase 2 (dsRNA Ligase) | E011 T4 RNA Ligase 2 (dsRNA Ligase) catalyzes the ATP-dependent formation of a 3'→5' phosphodiester bond catalyzing intramolecular and intermolecular RNA strand joining. Unlike T4 RNA Ligase 1 which ligates...

abm | T4 RNA Ligase 1 (ssRNA Ligase) | E010 T4 RNA Ligase 1 catalyzes the ATP-dependent formation of a 3'→5' phosphodiester bond on a broad range of substrates including single-stranded RNA and DNA, oligoribonucleotides, oligodeoxynucleotides, as well...

abm | T4 Polynucleotide Kinase | E006 T4 Polynucleotide Kinase catalyzes the transfer of the γ-phosphate from ATP to the 5´-hydroxyl terminus of double and single-stranded RNA and DNA, oligonucleotides or nucleoside 3´-monophosphates. The enzyme is also...

abm | T4 Gene 32 Protein | E040 T4 Gene 32 Protein is a single-stranded DNA (ssDNA) binding protein which is required for T4 bacteriophage replication, recombination and repair. T4 Gene 32 Protein binds cooperatively and stoichiometrically to ssDNA...