T4 Polynucleotide Kinase | E006
- 5 to 7 Days Shipment
abm | T4 Polynucleotide Kinase | E006
T4 Polynucleotide Kinase catalyzes the transfer of the γ-phosphate from ATP to the 5´-hydroxyl terminus of double and single-stranded RNA and DNA, oligonucleotides or nucleoside 3´-monophosphates. The enzyme is also capable of catalyzing the removal of 3´-phosphoryl groups from 3´-phosphoryl polynucleotides, deoxynucleoside 3´-monophosphates and deoxynucleoside 3´-diphosphates.
Enzyme supplied with 10X Reaction Buffer
One unit is defined as the amount of T4 Polynucleotide Kinase that catalyzes the incorporation of 1 nmol γ-phosphate from ATP to the 5’-hydroxyl termini of micrococcal nuclease-treated DNA in 30 minutes at 37°C in 1X T4 Polynucleotide Kinase Reaction Buffer.
This product is distributed for laboratory research only. Caution: Not for diagnostic use.
Single strand DNA nicking.
Store all components at -20°C.
10 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1 µM ATP, and 50% (v/v) Glycerol.
1000 U (100 μl)